Construct: shRNA TRCN0000077098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019737.1-3486s1c1
DNA Barcode:
GCCCAAATAGTGTTCTTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4galt6 (56386)
Gene Description:
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6
Transcript:
RefSeq NM_019737.1 (NON-CURRENT)
Match location:
Position 3486 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAAATAGTGTTCTTACTT
Hairpin Sequence:
5'-CCGG-GCCCAAATAGTGTTCTTACTT-CTCGAG-AAGTAAGAACACTATTTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAAATAGTGTTCTTACTTCTCGAGAAGTAAGAACACTATTTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAAATAGTGTTCTTACTTCTCGAGAAGTAAGAACACTATTTGGGC-3'

Other clones with same target sequence:

(none)