Construct: shRNA TRCN0000077140
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027769.2-1354s1c1
- DNA Barcode:
- GCAGACAGCTTCTCAATACTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cpne3 (70568)
- Gene Description:
- copine III
- Transcript:
- RefSeq NM_027769.2 (NON-CURRENT)
- Match location:
- Position 1354 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000077140)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGACAGCTTCTCAATACTT
- Hairpin Sequence:
-
5'-CCGG-GCAGACAGCTTCTCAATACTT-CTCGAG-AAGTATTGAGAAGCTGTCTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGACAGCTTCTCAATACTTCTCGAGAAGTATTGAGAAGCTGTCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGACAGCTTCTCAATACTTCTCGAGAAGTATTGAGAAGCTGTCTGC-3'
Other clones with same target sequence:
(none)
