Construct: shRNA TRCN0000077166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138599.2-694s1c1
DNA Barcode:
CTGTTGAACTTAATCCCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tomm70a (28185)
Gene Description:
translocase of outer mitochondrial membrane 70 homolog A (yeast)
Transcript:
RefSeq NM_138599.2 (NON-CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTTGAACTTAATCCCAAAT
Hairpin Sequence:
5'-CCGG-CTGTTGAACTTAATCCCAAAT-CTCGAG-ATTTGGGATTAAGTTCAACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTTGAACTTAATCCCAAATCTCGAGATTTGGGATTAAGTTCAACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTTGAACTTAATCCCAAATCTCGAGATTTGGGATTAAGTTCAACAG-3'

Other clones with same target sequence:

  1. TRCN0000317697