Construct: shRNA TRCN0000077204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011073.2-1297s1c1
DNA Barcode:
GCCCATTTGGTGGTAAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prf1 (18646)
Gene Description:
perforin 1 (pore forming protein)
Transcript:
RefSeq NM_011073.2 (NON-CURRENT)
Match location:
Position 1297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTTGGTGGTAAGCAAT
Hairpin Sequence:
5'-CCGG-GCCCATTTGGTGGTAAGCAAT-CTCGAG-ATTGCTTACCACCAAATGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTTGGTGGTAAGCAATCTCGAGATTGCTTACCACCAAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTTGGTGGTAAGCAATCTCGAGATTGCTTACCACCAAATGGGC-3'

Other clones with same target sequence:

(none)