Construct: shRNA TRCN0000077217

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007859.2-910s1c1
DNA Barcode:
ACCTGGAACCTGGATCATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dffb (13368)
Gene Description:
DNA fragmentation factor, beta subunit
Transcript:
RefSeq NM_007859.2 (NON-CURRENT)
Match location:
Position 910 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077217)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGGAACCTGGATCATATA
Hairpin Sequence:
5'-CCGG-ACCTGGAACCTGGATCATATA-CTCGAG-TATATGATCCAGGTTCCAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGGAACCTGGATCATATACTCGAGTATATGATCCAGGTTCCAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGGAACCTGGATCATATACTCGAGTATATGATCCAGGTTCCAGGT-3'

Other clones with same target sequence:

  1. TRCN0000331496