Construct: shRNA TRCN0000077240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009532.2-1930s1c1
DNA Barcode:
CCAGGACAATAGTGACACTGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrcc1 (22594)
Gene Description:
X-ray repair complementing defective repair in Chinese hamster cells 1
Transcript:
RefSeq NM_009532.2 (NON-CURRENT)
Match location:
Position 1930 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGACAATAGTGACACTGA
Hairpin Sequence:
5'-CCGG-CCAGGACAATAGTGACACTGA-CTCGAG-TCAGTGTCACTATTGTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGACAATAGTGACACTGACTCGAGTCAGTGTCACTATTGTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGACAATAGTGACACTGACTCGAGTCAGTGTCACTATTGTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000301394