Construct: shRNA TRCN0000077253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023210.2-1133s1c1
DNA Barcode:
GCGATCACATTGTCTTTGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Anp32e (66471)
Gene Description:
acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
Transcript:
RefSeq NM_023210.2 (NON-CURRENT)
Match location:
Position 1133 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATCACATTGTCTTTGTTT
Hairpin Sequence:
5'-CCGG-GCGATCACATTGTCTTTGTTT-CTCGAG-AAACAAAGACAATGTGATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATCACATTGTCTTTGTTTCTCGAGAAACAAAGACAATGTGATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATCACATTGTCTTTGTTTCTCGAGAAACAAAGACAATGTGATCGC-3'

Other clones with same target sequence:

  1. TRCN0000311769