Construct: shRNA TRCN0000077348
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_023324.1-2649s1c1
- DNA Barcode:
- GCTCCTTTGAATATGCAATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Peli1 (67245)
- Gene Description:
- pellino 1
- Transcript:
- RefSeq NM_023324.1 (NON-CURRENT)
- Match location:
- Position 2649 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000077348)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCCTTTGAATATGCAATTT
- Hairpin Sequence:
-
5'-CCGG-GCTCCTTTGAATATGCAATTT-CTCGAG-AAATTGCATATTCAAAGGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCCTTTGAATATGCAATTTCTCGAGAAATTGCATATTCAAAGGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCCTTTGAATATGCAATTTCTCGAGAAATTGCATATTCAAAGGAGC-3'
Other clones with same target sequence:
(none)
