Construct: shRNA TRCN0000077350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023324.1-1454s1c1
DNA Barcode:
GCTTTAAGACAGGAGATCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Peli1 (67245)
Gene Description:
pellino 1
Transcript:
RefSeq NM_023324.1 (NON-CURRENT)
Match location:
Position 1454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTAAGACAGGAGATCAAT
Hairpin Sequence:
5'-CCGG-GCTTTAAGACAGGAGATCAAT-CTCGAG-ATTGATCTCCTGTCTTAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTAAGACAGGAGATCAATCTCGAGATTGATCTCCTGTCTTAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTAAGACAGGAGATCAATCTCGAGATTGATCTCCTGTCTTAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000311836