Construct: shRNA TRCN0000077413

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011999.2-2020s1c1
DNA Barcode:
GCAGCATATTAGACACAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec4a2 (26888)
Gene Description:
C-type lectin domain family 4, member a2
Transcript:
RefSeq NM_011999.2 (NON-CURRENT)
Match location:
Position 2020 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077413)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCATATTAGACACAAGAT
Hairpin Sequence:
5'-CCGG-GCAGCATATTAGACACAAGAT-CTCGAG-ATCTTGTGTCTAATATGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCATATTAGACACAAGATCTCGAGATCTTGTGTCTAATATGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCATATTAGACACAAGATCTCGAGATCTTGTGTCTAATATGCTGC-3'

Other clones with same target sequence:

(none)