Construct: shRNA TRCN0000077478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177345.3-2053s1c1
DNA Barcode:
CCCATATTGAACAGGCAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapkap1 (227743)
Gene Description:
mitogen-activated protein kinase associated protein 1
Transcript:
RefSeq NM_177345.3 (NON-CURRENT)
Match location:
Position 2053 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATATTGAACAGGCAGATT
Hairpin Sequence:
5'-CCGG-CCCATATTGAACAGGCAGATT-CTCGAG-AATCTGCCTGTTCAATATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATATTGAACAGGCAGATTCTCGAGAATCTGCCTGTTCAATATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATATTGAACAGGCAGATTCTCGAGAATCTGCCTGTTCAATATGGG-3'

Other clones with same target sequence:

  1. TRCN0000301432