Construct: shRNA TRCN0000077485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146018.1-529s1c1
DNA Barcode:
CCAGGCTATATCAGTCATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Flcn (216805)
Gene Description:
folliculin
Transcript:
RefSeq NM_146018.1 (NON-CURRENT)
Match location:
Position 529 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCTATATCAGTCATGAT
Hairpin Sequence:
5'-CCGG-CCAGGCTATATCAGTCATGAT-CTCGAG-ATCATGACTGATATAGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCTATATCAGTCATGATCTCGAGATCATGACTGATATAGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCTATATCAGTCATGATCTCGAGATCATGACTGATATAGCCTGG-3'

Other clones with same target sequence:

(none)