Construct: shRNA TRCN0000077573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138956.2-2424s1c1
DNA Barcode:
CCCAGCTCTTAGCCTTTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf3 (192678)
Gene Description:
Ras association (RalGDS/AF-6) domain family member 3
Transcript:
RefSeq NM_138956.2 (NON-CURRENT)
Match location:
Position 2424 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCTCTTAGCCTTTCTAT
Hairpin Sequence:
5'-CCGG-CCCAGCTCTTAGCCTTTCTAT-CTCGAG-ATAGAAAGGCTAAGAGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCTCTTAGCCTTTCTATCTCGAGATAGAAAGGCTAAGAGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCTCTTAGCCTTTCTATCTCGAGATAGAAAGGCTAAGAGCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000316793