Construct: shRNA TRCN0000077592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011245.1-488s1c1
DNA Barcode:
CCGGCGAGAAACAGCAACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rasgrf1 (19417)
Gene Description:
RAS protein-specific guanine nucleotide-releasing factor 1
Transcript:
RefSeq NM_011245.1 (NON-CURRENT)
Match location:
Position 488 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGCGAGAAACAGCAACATT
Hairpin Sequence:
5'-CCGG-CCGGCGAGAAACAGCAACATT-CTCGAG-AATGTTGCTGTTTCTCGCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGCGAGAAACAGCAACATTCTCGAGAATGTTGCTGTTTCTCGCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGCGAGAAACAGCAACATTCTCGAGAATGTTGCTGTTTCTCGCCGG-3'

Other clones with same target sequence:

(none)