Construct: shRNA TRCN0000077647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175445.3-423s1c1
DNA Barcode:
GAAGTGGATATGCCAGTTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf2 (215653)
Gene Description:
Ras association (RalGDS/AF-6) domain family member 2
Transcript:
RefSeq NM_175445.3 (NON-CURRENT)
Match location:
Position 423 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGGATATGCCAGTTGAA
Hairpin Sequence:
5'-CCGG-GAAGTGGATATGCCAGTTGAA-CTCGAG-TTCAACTGGCATATCCACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGGATATGCCAGTTGAACTCGAGTTCAACTGGCATATCCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGGATATGCCAGTTGAACTCGAGTTCAACTGGCATATCCACTTC-3'

Other clones with same target sequence:

(none)