Construct: shRNA TRCN0000077714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018750.1-982s1c1
DNA Barcode:
CGGATACACAAAGATGGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rassf5 (54354)
Gene Description:
Ras association (RalGDS/AF-6) domain family member 5
Transcript:
RefSeq NM_018750.1 (NON-CURRENT)
Match location:
Position 982 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATACACAAAGATGGACAA
Hairpin Sequence:
5'-CCGG-CGGATACACAAAGATGGACAA-CTCGAG-TTGTCCATCTTTGTGTATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATACACAAAGATGGACAACTCGAGTTGTCCATCTTTGTGTATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATACACAAAGATGGACAACTCGAGTTGTCCATCTTTGTGTATCCG-3'

Other clones with same target sequence:

(none)