Construct: shRNA TRCN0000077752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025846.1-399s1c1
DNA Barcode:
ACGGACTATGATCCAACCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rras2 (66922)
Gene Description:
related RAS viral (r-ras) oncogene 2
Transcript:
RefSeq NM_025846.1 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGACTATGATCCAACCATT
Hairpin Sequence:
5'-CCGG-ACGGACTATGATCCAACCATT-CTCGAG-AATGGTTGGATCATAGTCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGACTATGATCCAACCATTCTCGAGAATGGTTGGATCATAGTCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGACTATGATCCAACCATTCTCGAGAATGGTTGGATCATAGTCCGT-3'

Other clones with same target sequence:

  1. TRCN0000326202