Construct: shRNA TRCN0000077827

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011806.2-664s1c1
DNA Barcode:
CTCACTAACAAAGGACATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dmtf1 (23857)
Gene Description:
cyclin D binding myb-like transcription factor 1
Transcript:
RefSeq NM_011806.2 (NON-CURRENT)
Match location:
Position 664 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077827)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACTAACAAAGGACATAAA
Hairpin Sequence:
5'-CCGG-CTCACTAACAAAGGACATAAA-CTCGAG-TTTATGTCCTTTGTTAGTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACTAACAAAGGACATAAACTCGAGTTTATGTCCTTTGTTAGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACTAACAAAGGACATAAACTCGAGTTTATGTCCTTTGTTAGTGAG-3'

Other clones with same target sequence:

(none)