Construct: shRNA TRCN0000077862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025252.2-548s1c1
DNA Barcode:
GCGTCAAATCACAGAAACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAPH1 (65059)
Gene Description:
Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Transcript:
RefSeq NM_025252.2 (NON-CURRENT)
Match location:
Position 548 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCAAATCACAGAAACGAA
Hairpin Sequence:
5'-CCGG-GCGTCAAATCACAGAAACGAA-CTCGAG-TTCGTTTCTGTGATTTGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCAAATCACAGAAACGAACTCGAGTTCGTTTCTGTGATTTGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCAAATCACAGAAACGAACTCGAGTTCGTTTCTGTGATTTGACGC-3'

Other clones with same target sequence:

(none)