Construct: shRNA TRCN0000077902

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178169.2-277s1c1
DNA Barcode:
GAGATCAAAGAGAAAGTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASSF3 (283349)
Gene Description:
Ras association domain family member 3
Transcript:
RefSeq NM_178169.2 (NON-CURRENT)
Match location:
Position 277 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077902)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATCAAAGAGAAAGTTCAT
Hairpin Sequence:
5'-CCGG-GAGATCAAAGAGAAAGTTCAT-CTCGAG-ATGAACTTTCTCTTTGATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATCAAAGAGAAAGTTCATCTCGAGATGAACTTTCTCTTTGATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATCAAAGAGAAAGTTCATCTCGAGATGAACTTTCTCTTTGATCTC-3'

Other clones with same target sequence:

(none)