Construct: shRNA TRCN0000077945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001553.1-738s1c1
DNA Barcode:
GTAAGGAAGATGCTGGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGFBP7 (3490)
Gene Description:
insulin like growth factor binding protein 7
Transcript:
RefSeq NM_001553.1 (NON-CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000077945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAGGAAGATGCTGGAGAAT
Hairpin Sequence:
5'-CCGG-GTAAGGAAGATGCTGGAGAAT-CTCGAG-ATTCTCCAGCATCTTCCTTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAGGAAGATGCTGGAGAATCTCGAGATTCTCCAGCATCTTCCTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAGGAAGATGCTGGAGAATCTCGAGATTCTCCAGCATCTTCCTTAC-3'

Other clones with same target sequence:

(none)