Construct: shRNA TRCN0000078074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015426.2-227s1c1
DNA Barcode:
CCTGTGTGAACTTCTCTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POC1A (25886)
Gene Description:
POC1 centriolar protein A
Transcript:
RefSeq NM_015426.2 (NON-CURRENT)
Match location:
Position 227 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTGAACTTCTCTCCTT
Hairpin Sequence:
5'-CCGG-CCTGTGTGAACTTCTCTCCTT-CTCGAG-AAGGAGAGAAGTTCACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTGAACTTCTCTCCTTCTCGAGAAGGAGAGAAGTTCACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTGAACTTCTCTCCTTCTCGAGAAGGAGAGAAGTTCACACAGG-3'

Other clones with same target sequence:

  1. TRCN0000289288