Construct: shRNA TRCN0000078115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006859.2-981s1c1
DNA Barcode:
GCTTGACTTTAGGACAATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIAS (11019)
Gene Description:
lipoic acid synthetase
Transcript:
RefSeq NM_006859.2 (NON-CURRENT)
Match location:
Position 981 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGACTTTAGGACAATATA
Hairpin Sequence:
5'-CCGG-GCTTGACTTTAGGACAATATA-CTCGAG-TATATTGTCCTAAAGTCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGACTTTAGGACAATATACTCGAGTATATTGTCCTAAAGTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGACTTTAGGACAATATACTCGAGTATATTGTCCTAAAGTCAAGC-3'

Other clones with same target sequence:

(none)