Construct: shRNA TRCN0000078168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006826.2-1826s1c1
DNA Barcode:
GCCCTATGTAACAGCAGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YWHAQ (10971)
Gene Description:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Transcript:
RefSeq NM_006826.2 (NON-CURRENT)
Match location:
Position 1826 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTATGTAACAGCAGAGTA
Hairpin Sequence:
5'-CCGG-GCCCTATGTAACAGCAGAGTA-CTCGAG-TACTCTGCTGTTACATAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTATGTAACAGCAGAGTACTCGAGTACTCTGCTGTTACATAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTATGTAACAGCAGAGTACTCGAGTACTCTGCTGTTACATAGGGC-3'

Other clones with same target sequence:

(none)