Construct: shRNA TRCN0000078181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_293875.4-558s1c1
DNA Barcode:
GCTGAAAGACATCTATGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCXD3 (345557)
Gene Description:
phosphatidylinositol specific phospholipase C X domain containing 3
Transcript:
RefSeq XM_293875.4 (NON-CURRENT)
Match location:
Position 558 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAAGACATCTATGGAAA
Hairpin Sequence:
5'-CCGG-GCTGAAAGACATCTATGGAAA-CTCGAG-TTTCCATAGATGTCTTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAAGACATCTATGGAAACTCGAGTTTCCATAGATGTCTTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAAGACATCTATGGAAACTCGAGTTTCCATAGATGTCTTTCAGC-3'

Other clones with same target sequence:

(none)