Construct: shRNA TRCN0000078265

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001902.4-836s1c1
DNA Barcode:
GCCTTCATAATAGACTTCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTH (1491)
Gene Description:
cystathionine gamma-lyase
Transcript:
RefSeq NM_001902.4 (NON-CURRENT)
Match location:
Position 836 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078265)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCATAATAGACTTCGTT
Hairpin Sequence:
5'-CCGG-GCCTTCATAATAGACTTCGTT-CTCGAG-AACGAAGTCTATTATGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCATAATAGACTTCGTTCTCGAGAACGAAGTCTATTATGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCATAATAGACTTCGTTCTCGAGAACGAAGTCTATTATGAAGGC-3'

Other clones with same target sequence:

(none)