Construct: shRNA TRCN0000078371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018417.2-2486s1c1
DNA Barcode:
CCTGTTCAAGTATTCCATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADCY10 (55811)
Gene Description:
adenylate cyclase 10
Transcript:
RefSeq NM_018417.2 (NON-CURRENT)
Match location:
Position 2486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTTCAAGTATTCCATTAA
Hairpin Sequence:
5'-CCGG-CCTGTTCAAGTATTCCATTAA-CTCGAG-TTAATGGAATACTTGAACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTTCAAGTATTCCATTAACTCGAGTTAATGGAATACTTGAACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTTCAAGTATTCCATTAACTCGAGTTAATGGAATACTTGAACAGG-3'

Other clones with same target sequence:

(none)