Construct: shRNA TRCN0000078375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001117.2-481s1c1
DNA Barcode:
GAGGTATAAACAAAGGGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADCYAP1 (116)
Gene Description:
adenylate cyclase activating polypeptide 1
Transcript:
RefSeq NM_001117.2 (NON-CURRENT)
Match location:
Position 481 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGTATAAACAAAGGGTTAA
Hairpin Sequence:
5'-CCGG-GAGGTATAAACAAAGGGTTAA-CTCGAG-TTAACCCTTTGTTTATACCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGTATAAACAAAGGGTTAACTCGAGTTAACCCTTTGTTTATACCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGTATAAACAAAGGGTTAACTCGAGTTAACCCTTTGTTTATACCTC-3'

Other clones with same target sequence:

(none)