Construct: shRNA TRCN0000078378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005772.2-1844s1c1
DNA Barcode:
CGGATCTTCATCTGGTTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RCL1 (10171)
Gene Description:
RNA terminal phosphate cyclase like 1
Transcript:
RefSeq NM_005772.2 (NON-CURRENT)
Match location:
Position 1844 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATCTTCATCTGGTTCATT
Hairpin Sequence:
5'-CCGG-CGGATCTTCATCTGGTTCATT-CTCGAG-AATGAACCAGATGAAGATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATCTTCATCTGGTTCATTCTCGAGAATGAACCAGATGAAGATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATCTTCATCTGGTTCATTCTCGAGAATGAACCAGATGAAGATCCG-3'

Other clones with same target sequence:

(none)