Construct: shRNA TRCN0000078388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006366.2-2043s1c1
DNA Barcode:
CCTTTGAGAATCTAAGATGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAP2 (10486)
Gene Description:
cyclase associated actin cytoskeleton regulatory protein 2
Transcript:
RefSeq NM_006366.2 (NON-CURRENT)
Match location:
Position 2043 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGAGAATCTAAGATGTA
Hairpin Sequence:
5'-CCGG-CCTTTGAGAATCTAAGATGTA-CTCGAG-TACATCTTAGATTCTCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGAGAATCTAAGATGTACTCGAGTACATCTTAGATTCTCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGAGAATCTAAGATGTACTCGAGTACATCTTAGATTCTCAAAGG-3'

Other clones with same target sequence:

(none)