Construct: shRNA TRCN0000078428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000532.2-1520s1c1
DNA Barcode:
GCAGAGTACATCGAGAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCCB (5096)
Gene Description:
propionyl-CoA carboxylase subunit beta
Transcript:
RefSeq NM_000532.2 (NON-CURRENT)
Match location:
Position 1520 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGTACATCGAGAAGTTT
Hairpin Sequence:
5'-CCGG-GCAGAGTACATCGAGAAGTTT-CTCGAG-AAACTTCTCGATGTACTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGTACATCGAGAAGTTTCTCGAGAAACTTCTCGATGTACTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGTACATCGAGAAGTTTCTCGAGAAACTTCTCGATGTACTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000333347