Construct: shRNA TRCN0000078477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000818.1-667s1c1
DNA Barcode:
CCTAGATACTTCAATCAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAD2 (2572)
Gene Description:
glutamate decarboxylase 2
Transcript:
RefSeq NM_000818.1 (NON-CURRENT)
Match location:
Position 667 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGATACTTCAATCAACTT
Hairpin Sequence:
5'-CCGG-CCTAGATACTTCAATCAACTT-CTCGAG-AAGTTGATTGAAGTATCTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGATACTTCAATCAACTTCTCGAGAAGTTGATTGAAGTATCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGATACTTCAATCAACTTCTCGAGAAGTTGATTGAAGTATCTAGG-3'

Other clones with same target sequence:

(none)