Construct: shRNA TRCN0000078506

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015878.3-1321s1c1
DNA Barcode:
GCTTGCAAAGAATCTCAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AZIN1 (51582)
Gene Description:
antizyme inhibitor 1
Transcript:
RefSeq NM_015878.3 (NON-CURRENT)
Match location:
Position 1321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078506)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGCAAAGAATCTCAAGTA
Hairpin Sequence:
5'-CCGG-GCTTGCAAAGAATCTCAAGTA-CTCGAG-TACTTGAGATTCTTTGCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGCAAAGAATCTCAAGTACTCGAGTACTTGAGATTCTTTGCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGCAAAGAATCTCAAGTACTCGAGTACTTGAGATTCTTTGCAAGC-3'

Other clones with same target sequence:

(none)