Construct: shRNA TRCN0000078548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000535.2-1686s1c1
DNA Barcode:
CCAGGAAGATACCGGATGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PMS2 (5395)
Gene Description:
PMS1 homolog 2, mismatch repair system component
Transcript:
RefSeq NM_000535.2 (NON-CURRENT)
Match location:
Position 1686 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAAGATACCGGATGTAA
Hairpin Sequence:
5'-CCGG-CCAGGAAGATACCGGATGTAA-CTCGAG-TTACATCCGGTATCTTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAAGATACCGGATGTAACTCGAGTTACATCCGGTATCTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAAGATACCGGATGTAACTCGAGTTACATCCGGTATCTTCCTGG-3'

Other clones with same target sequence:

(none)