Construct: shRNA TRCN0000078629

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030648.1-725s1c1
DNA Barcode:
CCGCACTTTATGGGAAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SETD7 (80854)
Gene Description:
SET domain containing 7, histone lysine methyltransferase
Transcript:
RefSeq NM_030648.1 (NON-CURRENT)
Match location:
Position 725 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078629)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCACTTTATGGGAAATTTA
Hairpin Sequence:
5'-CCGG-CCGCACTTTATGGGAAATTTA-CTCGAG-TAAATTTCCCATAAAGTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCACTTTATGGGAAATTTACTCGAGTAAATTTCCCATAAAGTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCACTTTATGGGAAATTTACTCGAGTAAATTTCCCATAAAGTGCGG-3'

Other clones with same target sequence:

(none)