Construct: shRNA TRCN0000078681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152572.2-700s1c1
DNA Barcode:
GCACTTGCATAGAGACAACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK8 (158067)
Gene Description:
adenylate kinase 8
Transcript:
RefSeq NM_152572.2 (NON-CURRENT)
Match location:
Position 700 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTGCATAGAGACAACGA
Hairpin Sequence:
5'-CCGG-GCACTTGCATAGAGACAACGA-CTCGAG-TCGTTGTCTCTATGCAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTGCATAGAGACAACGACTCGAGTCGTTGTCTCTATGCAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTGCATAGAGACAACGACTCGAGTCGTTGTCTCTATGCAAGTGC-3'

Other clones with same target sequence:

(none)