Construct: shRNA TRCN0000078699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004742.2-1065s1c1
DNA Barcode:
GCACCTATGAAGGAAACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGI1 (9223)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 1
Transcript:
RefSeq NM_004742.2 (NON-CURRENT)
Match location:
Position 1065 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTATGAAGGAAACTATT
Hairpin Sequence:
5'-CCGG-GCACCTATGAAGGAAACTATT-CTCGAG-AATAGTTTCCTTCATAGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTATGAAGGAAACTATTCTCGAGAATAGTTTCCTTCATAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTATGAAGGAAACTATTCTCGAGAATAGTTTCCTTCATAGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000299723