Construct: shRNA TRCN0000078718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080860.2-1048s1c1
DNA Barcode:
CGAGAGGAGATCGTATCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSPH1 (89765)
Gene Description:
radial spoke head component 1
Transcript:
RefSeq NM_080860.2 (NON-CURRENT)
Match location:
Position 1048 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAGGAGATCGTATCATAA
Hairpin Sequence:
5'-CCGG-CGAGAGGAGATCGTATCATAA-CTCGAG-TTATGATACGATCTCCTCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAGGAGATCGTATCATAACTCGAGTTATGATACGATCTCCTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAGGAGATCGTATCATAACTCGAGTTATGATACGATCTCCTCTCG-3'

Other clones with same target sequence:

(none)