Construct: shRNA TRCN0000078752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025290.2-946s1c1
DNA Barcode:
GCTACGACATAGACCAGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rsph1 (22092)
Gene Description:
radial spoke head 1 homolog (Chlamydomonas)
Transcript:
RefSeq NM_025290.2 (NON-CURRENT)
Match location:
Position 946 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGACATAGACCAGGGAA
Hairpin Sequence:
5'-CCGG-GCTACGACATAGACCAGGGAA-CTCGAG-TTCCCTGGTCTATGTCGTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGACATAGACCAGGGAACTCGAGTTCCCTGGTCTATGTCGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGACATAGACCAGGGAACTCGAGTTCCCTGGTCTATGTCGTAGC-3'

Other clones with same target sequence:

(none)