Construct: shRNA TRCN0000078775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_149784.4-4461s1c1
DNA Barcode:
CCGGAACTTGAACGTGTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxnd1 (67784)
Gene Description:
plexin D1
Transcript:
RefSeq XM_149784.4 (NON-CURRENT)
Match location:
Position 4461 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAACTTGAACGTGTCCTT
Hairpin Sequence:
5'-CCGG-CCGGAACTTGAACGTGTCCTT-CTCGAG-AAGGACACGTTCAAGTTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAACTTGAACGTGTCCTTCTCGAGAAGGACACGTTCAAGTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAACTTGAACGTGTCCTTCTCGAGAAGGACACGTTCAAGTTCCGG-3'

Other clones with same target sequence:

(none)