Construct: shRNA TRCN0000078784

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_205324.2-2381s1c1
DNA Barcode:
CCACCAACTTAGATGTAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slitrk2 (245450)
Gene Description:
SLIT and NTRK-like family, member 2
Transcript:
RefSeq XM_205324.2 (NON-CURRENT)
Match location:
Position 2381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078784)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCAACTTAGATGTAAGTT
Hairpin Sequence:
5'-CCGG-CCACCAACTTAGATGTAAGTT-CTCGAG-AACTTACATCTAAGTTGGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCAACTTAGATGTAAGTTCTCGAGAACTTACATCTAAGTTGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCAACTTAGATGTAAGTTCTCGAGAACTTACATCTAAGTTGGTGG-3'

Other clones with same target sequence:

(none)