Construct: shRNA TRCN0000078801

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_487772.1-977s1c1
DNA Barcode:
CCAAGGAAATTGTCCACGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnni3k (435766)
Gene Description:
TNNI3 interacting kinase
Transcript:
RefSeq XM_487772.1 (NON-CURRENT)
Match location:
Position 977 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078801)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGAAATTGTCCACGTAA
Hairpin Sequence:
5'-CCGG-CCAAGGAAATTGTCCACGTAA-CTCGAG-TTACGTGGACAATTTCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGAAATTGTCCACGTAACTCGAGTTACGTGGACAATTTCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGAAATTGTCCACGTAACTCGAGTTACGTGGACAATTTCCTTGG-3'

Other clones with same target sequence:

(none)