Construct: shRNA TRCN0000078811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172665.1-852s1c1
DNA Barcode:
GCGGCTTTGTGATTTGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdk1 (228026)
Gene Description:
pyruvate dehydrogenase kinase, isoenzyme 1
Transcript:
RefSeq NM_172665.1 (NON-CURRENT)
Match location:
Position 852 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTTTGTGATTTGTATTA
Hairpin Sequence:
5'-CCGG-GCGGCTTTGTGATTTGTATTA-CTCGAG-TAATACAAATCACAAAGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTTTGTGATTTGTATTACTCGAGTAATACAAATCACAAAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTTTGTGATTTGTATTACTCGAGTAATACAAATCACAAAGCCGC-3'

Other clones with same target sequence:

(none)