Construct: shRNA TRCN0000078840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145401.1-1909s1c1
DNA Barcode:
GCAGATAGCATTGTGGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkag2 (108099)
Gene Description:
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Transcript:
RefSeq NM_145401.1 (NON-CURRENT)
Match location:
Position 1909 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATAGCATTGTGGGTATT
Hairpin Sequence:
5'-CCGG-GCAGATAGCATTGTGGGTATT-CTCGAG-AATACCCACAATGCTATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATAGCATTGTGGGTATTCTCGAGAATACCCACAATGCTATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATAGCATTGTGGGTATTCTCGAGAATACCCACAATGCTATCTGC-3'

Other clones with same target sequence:

(none)