Construct: shRNA TRCN0000078841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145401.1-1249s1c1
DNA Barcode:
CTCACGATTACAGATTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkag2 (108099)
Gene Description:
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Transcript:
RefSeq NM_145401.1 (NON-CURRENT)
Match location:
Position 1249 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACGATTACAGATTTCATA
Hairpin Sequence:
5'-CCGG-CTCACGATTACAGATTTCATA-CTCGAG-TATGAAATCTGTAATCGTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACGATTACAGATTTCATACTCGAGTATGAAATCTGTAATCGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACGATTACAGATTTCATACTCGAGTATGAAATCTGTAATCGTGAG-3'

Other clones with same target sequence:

(none)