Construct: shRNA TRCN0000078853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484491.2-5953s1c1
DNA Barcode:
GCGTCTTTCTCCTGAGCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxnb2 (140570)
Gene Description:
plexin B2
Transcript:
RefSeq XM_484491.2 (NON-CURRENT)
Match location:
Position 5953 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTTTCTCCTGAGCAATA
Hairpin Sequence:
5'-CCGG-GCGTCTTTCTCCTGAGCAATA-CTCGAG-TATTGCTCAGGAGAAAGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTTTCTCCTGAGCAATACTCGAGTATTGCTCAGGAGAAAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTTTCTCCTGAGCAATACTCGAGTATTGCTCAGGAGAAAGACGC-3'

Other clones with same target sequence:

(none)