Construct: shRNA TRCN0000078874
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133780.2-1154s1c1
- DNA Barcode:
- GCTCACATAAAGCTCTCTAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Tpr (108989)
- Gene Description:
- translocated promoter region, nuclear basket protein
- Transcript:
- RefSeq NM_133780.2 (NON-CURRENT)
- Match location:
- Position 1154 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000078874)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCACATAAAGCTCTCTAAT
- Hairpin Sequence:
-
5'-CCGG-GCTCACATAAAGCTCTCTAAT-CTCGAG-ATTAGAGAGCTTTATGTGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCACATAAAGCTCTCTAATCTCGAGATTAGAGAGCTTTATGTGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCACATAAAGCTCTCTAATCTCGAGATTAGAGAGCTTTATGTGAGC-3'
Other clones with same target sequence:
(none)
