Construct: shRNA TRCN0000078900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178775.3-1408s1c1
DNA Barcode:
GCAACGAGTATGGGCAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6kc1 (320119)
Gene Description:
ribosomal protein S6 kinase polypeptide 1
Transcript:
RefSeq NM_178775.3 (NON-CURRENT)
Match location:
Position 1408 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACGAGTATGGGCAAGAAA
Hairpin Sequence:
5'-CCGG-GCAACGAGTATGGGCAAGAAA-CTCGAG-TTTCTTGCCCATACTCGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACGAGTATGGGCAAGAAACTCGAGTTTCTTGCCCATACTCGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACGAGTATGGGCAAGAAACTCGAGTTTCTTGCCCATACTCGTTGC-3'

Other clones with same target sequence:

(none)