Construct: shRNA TRCN0000078959

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144854.1-506s1c1
DNA Barcode:
CTGCCAAATAGCAGAAGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map3k7cl (224419)
Gene Description:
Map3k7 C-terminal like
Transcript:
RefSeq NM_144854.1 (NON-CURRENT)
Match location:
Position 506 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000078959)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGCCAAATAGCAGAAGAGTA
Hairpin Sequence:
5'-CCGG-CTGCCAAATAGCAGAAGAGTA-CTCGAG-TACTCTTCTGCTATTTGGCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGCCAAATAGCAGAAGAGTACTCGAGTACTCTTCTGCTATTTGGCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGCCAAATAGCAGAAGAGTACTCGAGTACTCTTCTGCTATTTGGCAG-3'

Other clones with same target sequence:

(none)