Construct: shRNA TRCN0000079011

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_487131.1-271s1c1
DNA Barcode:
TCGGGAGGGAACTTATTTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC435230 (435230)
Gene Description:
similar to hypothetical protein 4930509O22
Transcript:
RefSeq XM_487131.1 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000079011)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGGAGGGAACTTATTTGAT
Hairpin Sequence:
5'-CCGG-TCGGGAGGGAACTTATTTGAT-CTCGAG-ATCAAATAAGTTCCCTCCCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGGAGGGAACTTATTTGATCTCGAGATCAAATAAGTTCCCTCCCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGGAGGGAACTTATTTGATCTCGAGATCAAATAAGTTCCCTCCCGA-3'

Other clones with same target sequence:

(none)